Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.

نویسندگان

  • A A Dutra
  • L O Sousa
  • R R Resende
  • R L Brandão
  • E Kalapothakis
  • I M Castro
چکیده

Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular cloning of toxins expressed by the venom gland of Lasiodora sp.

The present work describes the identification of toxins expressed by the venom gland of the spider Lasiodora sp. The toxins LTx1, LTx2 and LTx3 were identified by the screening of a cDNA library. These toxins showed significant similarity at the amino acid level with spider toxins from Lasiodora parahybana, Eurypelma californicum, Brachypelma smithii, Selenocosmia huwena.

متن کامل

Regulatory Role of Calcium Channel Blockers on Spontaneous Muscular Activity of Gastrothylax Crumenifer, A Rumen Amphistome

Major proportion of intracellular calcium (Ca 2+ ) is achieved through opening of calcium channels present in the plasma membrane which play an important role in regulating neuromuscular coordination and release of neurotransmitters from nerve terminals. Blockade of these calcium channels adversely affect contractile process and release of neurotransmitters in majority of the neuromuscular prep...

متن کامل

Expression and Purification of Neurotoxin-Associated Protein HA-33/A from Clostridium botulinum and Evaluation of Its Antigenicity

Background: Botulinum neurotoxin (BoNT) complexes consist of neurotoxin and neurotoxin-associated proteins. Hemagglutinin-33 (HA-33) is a member of BoNT type A (BoNT/A) complex. Considering the protective role of HA-33 in preservation of BoNT/A in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. Thus in this study, HA-33 was expr...

متن کامل

P-94: The Effect of Calcium Ionophore A23187 and Ethanol on Parthenogenetic Activation of Mouse Oocytes in Presence of Hydrostatic Pressure and Cy-tochalasin B

Background: Parthenogenetic activation of mammalian oocytes using artificial stimuli is commonly used in various reproductive bio-techniques. Calcium ionophore is known to elevate intracellular calcium levels in the cytoplasm of oocytes through the influx of calcium from extracellular spaces. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial ...

متن کامل

The Effect of Cinnamon Hydroalcoholic Extract on the Expression of Sperm Calcium Channels (Catsper) Genes in Male Rats Receiving a High-fat Diet

Introduction: Cinnamon is a plant containing anti-inflammatory, anti-apoptotic, and antioxidant substances. Previous studies have revealed the reducing effects of obesity and hyperlipidemia and, on the contrary, the reinforcing effects of cinnamon on fertility. Therefore in this study the effect of the hydroalcoholic extract of cinnamon on Body Mass Index (BMI) and gene expression of cation cha...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Peptides

دوره 29 9  شماره 

صفحات  -

تاریخ انتشار 2008